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【COVID-19 Research Achievement】New microfluidic cartridge for single-molecule droplet polymerase chain reaction (PCR)

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Updated Mar. 17, 2021
Masahiko Hashimoto (Professor, Faculty of Science and Engineering) and his colleagues have developed a new microfluidic cartridge for single-molecule droplet polymerase chain reaction (PCR). The new cartridge eliminates the need for manual droplet transfer between the major steps of droplet PCR analysis, allowing very convenient single-molecule droplet PCR. They successfully detected single synthetic RNA molecules of SARS-CoV-2 using the cartridge and common laboratory equipment.

Research Group:
Masahiko Hashimoto
(Professor, Faculty of Science and Engineering)
Hirokazu Takahara (Graduate School of Science and Engineering)
Hiroo Matsushita (same as above)
Erika Inui (same as above)
Masashi Ochiai (same as above)

The article about this research achievement (below) has been published in the Analytical Methods of the Royal Society of Chemistry, UK, and selected as the Outside Front Cover.
“Convenient microfluidic cartridge for single-molecule droplet PCR using common laboratory equipment” (Analytical Methods 13(8), 28 February 2021, pp.974-985, doi: 10.1039/d0ay01779e)
Masahiko Hashimoto (Professor, Faculty of Science and Engineering) and his colleagues have developed a new microfluidic cartridge for single-molecule droplet polymerase chain reaction (PCR). The new cartridge eliminates the need for manual droplet transfer between the major steps of droplet PCR analysis, allowing very convenient single-molecule droplet PCR. They successfully detected single synthetic RNA molecules of SARS-CoV-2 using the cartridge and common laboratory equipment.

Research Group:
Masahiko Hashimoto
(Professor, Faculty of Science and Engineering)
Hirokazu Takahara (Graduate School of Science and Engineering)
Hiroo Matsushita (same as above)
Erika Inui (same as above)
Masashi Ochiai (same as above)

The article about this research achievement (below) has been published in the Analytical Methods of the Royal Society of Chemistry, UK, and selected as the Outside Front Cover.
“Convenient microfluidic cartridge for single-molecule droplet PCR using common laboratory equipment” (Analytical Methods 13(8), 28 February 2021, pp.974-985, doi: 10.1039/d0ay01779e)
About the pictures:
(A)
Photographic image of the developed microfluidic cartridge (PDMS part).
(B)
Fluorescence image of PCR-amplified droplets. The bright spots indicate the droplets containing PCR amplicons derived from single synthetic RNA molecules of SARS-CoV-2.
cartridge

(A)

fluorescence

(B)

About the pictures:
(A)
Photographic image of the developed microfluidic cartridge (PDMS part).
(B)
Fluorescence image of PCR-amplified droplets. The bright spots indicate the droplets containing PCR amplicons derived from single synthetic RNA molecules of SARS-CoV-2.
Related Information
Contact
Department of Research Planning
E-mail:ji-knkak@mail.doshisha.ac.jp